Journal: JBMR Plus
Article Title: β 2 -adrenoceptor signaling in chondrocytes regulates long bone growth, bone homeostasis, and fracture healing in mice
doi: 10.1093/jbmrpl/ziag021
Figure Lengend Snippet: Long bone growth of 12-wk-old male Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) collagen 2 + chondrocytes and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05, ** p < .01, **** p < .0001.
Article Snippet: For immunofluorescence double staining of Collagen 2 (goat anti-mouse Col2, 1:50, #1320-01, Southern Biotech) and β 2 -AR (rabbit anti-mouse β 2 -AR, 1:50, #PA5-77283, Thermo Fisher Scientific), decalcified sections of intact and fractured femora were used.
Techniques: Immunohistochemical staining, Staining